人类发育过程中,各个器官都要经过复杂的形态发生和组织分化, 逐渐获得特定的形态并执行一定的生理功能。
各种各样的临床因素会在器官、组织发育和应对损伤的过程中引发炎症、纤维化和肿瘤等疾病。
以肺的纤维化和肿瘤为例,要准确区分肺纤维化的形式才能给予患者有效治疗。
结缔组织疾病引发的肺纤维化可以接受免疫抑制治疗,但对于特发性肺纤维化
(IPF)
来说,免疫抑制治疗效果有限。
而肿瘤对药物暴露的反应在很大程度上受到肿瘤细胞生长的肿瘤微环境
(TME)
的组成和活性状态的影响。
TME是复杂且异质性的,由无数种细胞类型组成,包括肿瘤、免疫、基质和血管内皮细胞,它们与非细胞成分如细胞外基质交织在一起,这对新的研究性抗癌疗法的临床前评估和开发提出了重大挑战。
RNAscope
TM
技术作为RNA ISH的金标准,能够为各种疾病的研究与病理诊断提供便捷强大的支持。该技术由Bio-Techne旗下Advanced Cell Diagnostics
(ACD
TM
)公司研发,在近年来的生物检测领域发展迅速。与传统的RNA原位杂交相比,其特异性的“双Z探针”设计避免了传统长链RNA探针对样本RNA降解非常敏感的弊端,配以自身级联放大检测,能放大特异性信号而非噪音信号,在单分子水平高效敏感地检测到多个目标RNA,且能在同一张切片上同时检测蛋白靶标。其操作流程非常简单,适用于任意种属、任意组织、任意RNA的原位检测。
Bio-Techne
(NASDAQ:TECH)
,致力于为生命科学和临床诊断研究人员提供高品质的试剂、仪器、定制生产和检测服务。2007年,在上海成立中国分公司,助力中国生命科学事业的发展与腾飞,目前旗下拥有 R&D Systems
TM
、Novus Biologicals
TM
、Tocris
TM
、ProteinSimple
TM
、PrimeGene
TM
和ACD
TM
等众多一线品牌,形成了独特的产品和服务组合。
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